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microDisruptor 24 Pack
microDisruptor 24 Pack
 
Price (Without Additional Items):$221.00

: Catalog Number: 04.250.24
Product Code: 04.250.24

Description
 

Tissue Homogenizers

microDisruptor™ 24 Pack - With Battery Pack

ClaremontBio is pleased to offer microDisruptor as an afforable alternative to larger bench-top homogenizers in a very compact and disposable device. The microDisruptor is 1.5" tall and is capable of homogenizing leaf punches and tissue samples in minutes without the bulky equipment and can be used in the field. This kit includes 24 microDisruptor and comes with a 1.5 volt Bat-Pac 1xAAA battery pack.


Peer-Reviewed Citations & References:
microDisruptor Citations and References


PROTOCOL:

1. Place 200-400ul of your solution of choice into disruption chamber. Samples can consist of small pieces of animal tissue (20-30mg), leaf punches (3-6mm diameter), or a solution of hard-to-disrupt cells (greater than 109cells/ml; use of 0.1-0.2mm beads suggested).

2. If desired, tissue or plant samples can be disrupted with the help additional beads (0.5mm beads are provided for this use). However, disruption of tissue has been shown to be achieved without the use of beads. As an option, the user may choose to initially shred tissue within the chamber without using beads and then decide to add beads (0.1-0.5mm) to further disrupt intact cells or provide finer tissue homogenization.

3. Activate the microHomogenizerTM or microDisruptorTM by moving the switch on the Bat-PacTM to the “On” position. Occasionally shake the chamber during operation to help any undisrupted chunks into solution. If using the microHomogenizerTM, the motor can be easily raised, lowered, or tilted within the included microfuge tube to achieve optimal disruption. If using the microHomogenizerTM, you may find that it is necessary to tilt the handle of the device such that the impeller of the motor is not in contact with the side of the tube (preventing the motor from moving).

4. Disruption times will vary depending on desired outcome. For easy and efficient separation of lysate from the beads (if used), try our PrepTipTM recovery tips.

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