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  microHomogenizer 6 Pack

 
Our Price:$72.00
Sale Price:$36.00
You save $36.00!

Availability:: Catalog Number: 04.270.06
Product Code: 04.270.06

Description
 

Tissue Homogenizers

microHomogenizer™ 6 Pack - With Battery Pack


The microHomogenizer™ kits offers all the exceptional benefits of microDisruptor™ homogenizers in a convenient  microfuge tube format. This kit is completely disposable and eliminates the need for lab space, making microHomogenizer perfect for use in the field as well as an excellent alternative for researcher bench tops cluttered with bulky homogenizers. The microHomogenizer comes with the lysing media, microfuge tubes, and a 1.5 volt Bat-Pac™ 1xAAA battery pack.
Protocol
  • microHomogenizer™ Tissue Homogenization Protocol

    You can view the PDF version of this protocol here: Tissue Homogenization Protocol

    Place
  • 200-400ul of your solution of choice into disruption chamber.  Samples
  • can consist of small pieces of animal tissue (20-30mg), leaf punches
  • (3-6mm diameter), or a solution of hard-to-disrupt cells (greater than
  • 1x109 cells/ml; use of 0.1-0.2mm beads suggested).
     
    2.
  • If desired, tissue or plant samples can be disrupted with the help
  • additional beads (0.5mm beads are provided for this use).  However,
  • disruption of tissue has been shown to be achieved without the use of
  • beads.  As an option, the user may choose to initially shred tissue
  • within the chamber without using beads and then decide to add beads
  • (0.1-0.5mm) to further disrupt intact cells or provide finer tissue
  • homogenization.
     
    3. Activate the microHomogenizer™ or
  • microDisruptor™ by moving the switch on the Bat-Pac™ to the “On”
  • position. Occasionally shake the chamber during operation to help any
  • undisrupted chunks into solution. If using the microHomogenizer™, the
  • motor can be easily raised, lowered, or tilted within the included
  • microfuge tube to achieve optimal disruption. If using the
  • microHomogenizer™, you may find that it is necessary to tilt the handle
  • of the device such that the impellor of the motor is not in contact with
  • the side of the tube (preventing the motor from moving).

    4.
  • Disruption times will vary depending on desired outcome.  For easy and
  • efficient separation of lysate from the beads (if used), try our
  • PrepTip™ recovery tips.

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