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OmniLyse® Inside Kits

Hard-To-Lyse Sample Prep in Minutes

Core Technology

  • Miniaturized mechanical lysis
  • Proven for hard-to-lyse microbes
  • Rapid & highly efficient
  • Easy-to-use & portable
  • Unique flow-through format
  • Low-power, -heat, -noise, -volume
  • Disposable (RoHS Compliant)
  • Multiple US & Intl. patents

Applications

  • Effective for mouse heart, tail, liver, brain, and plant tissues
  • Quick and convenient sample preparation in minutes
  • No need for lengthy overnight tissue digestion
  • Versatile for downstream applications
  • Suitable for DNA, RNA, and protein prep

Extraction Benefits

  • Rapidly extracts gDNA from bacteria in minutes
  • Compatible with various sample types
  • Optimized for purity and yield

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"OmniLyse methods provided the greatest consistent yield of genomic DNA."- Irwin et al. BMC Microbiology (2014) 14:326 - USDA publication - Near-Quantitative Sampling of Genomic DNA from Various Food-Borne Eubacteria


Cell Lysis for Hard-to-Lyse Organisms

OmniLyse Inside is specifically engineered for reliable disruption of hard-to-lyse organisms, including fungi, yeast, gram-positive bacteria, and other structurally resilient microbial cells. These organisms often resist traditional lysis methods, leading to incomplete disruption and inconsistent downstream results.

By delivering rapid and consistent mechanical lysis, OmniLyse ensures efficient release of nucleic acids, improving the quality and reproducibility of DNA and RNA extraction workflows.

Designed for Difficult Sample Types

Standard chemical and enzymatic lysis approaches frequently struggle with tough cell walls and complex sample matrices. OmniLyse overcomes these limitations with a mechanical lysis method optimized for challenging organisms, providing uniform and reproducible results.

  • Effective lysis of fungi, yeast, and gram-positive bacteria
  • Consistent disruption of tough and resilient cell walls
  • Improved DNA and RNA yield from difficult-to-process samples
  • Rapid processing,typically seconds to minutes
  • Compatible with downstream genomic and proteomic workflows

Applications in Advanced Workflows

OmniLyse Inside is widely used in workflows where conventional lysis methods fall short, including:

  • Fungal DNA and RNA extraction
  • Microbial diversity and microbiome studies
  • Environmental and soil sample preparation
  • Clinical and diagnostic pathogen detection
  • Sample preparation for next-generation sequencing (NGS)

Why Mechanical Lysis Matters

For hard-to-lyse organisms, mechanical disruption provides a more reliable solution than chemical lysis alone. OmniLyse delivers consistent shear forces to break down even the most resilient cell structures, enabling dependable results across a wide range of organisms and sample types.

A Proven Solution for Challenging Samples

OmniLyse technology has been validated across a wide range of difficult organisms and complex matrices. For researchers working with fungi, spores, gram-positive bacteria, and other resistant cell types, OmniLyse provides a dependable, high-performance solution for efficient sample preparation.


Patents and Intellectual Property

OmniLyse technology and related extraction methods are protected by a robust portfolio of issued patents worldwide.

US 8,663,974 (issued Mar 4, 2014)
JP 5663311 (issued Dec 12, 2014)
JP 5711227 (issued Mar 13, 2015)
US 9,260,475 (issued Feb 16, 2016)
US 9,428,725 (issued Aug 30, 2016)
EP 2232259 (issued Oct 12, 2016)
JP 6040268 (issued Nov 11, 2016)
AU 2010266034 (issued Mar 30, 2017)
EP 2446047 (issued Oct 18, 2017)
US 9,873,860 (issued Jan 23, 2018)
HK 1171052 (issued Aug 3, 2018)
US D829,339 (issued Sep 25, 2018)
US 10,428,301 (issued Oct 1, 2019)
US 10,801,001 (issued Oct 13, 2020)
US 11,286,447 (issued Mar 29, 2022)
US 11,286,447 (issued Oct 18, 2022)
US 11,479,747 (issued Oct 25, 2022)
CA 2,711,854 (issued Mar 21, 2023)

Additional US and international patents pending.


Protocols

OmniLyse Ultra-rapid Cell Lysis Protocol


Data & Images:


Peer-Reviewed Publications Referencing OmniLyse®

OmniLyse technology has been validated across a wide range of peer-reviewed publications, demonstrating consistent, high-efficiency lysis of hard-to-lyse organisms including fungi, gram-positive bacteria, and spores.


Near-Quantitative Sampling of Genomic DNA from Various Food-Borne Eubacteria

Bead Beating-Based Continuous Flow Cell Lysis in a Microfluidic Device

Aminoglycoside-modifying Enzymes Determine the Innate Susceptibility to Aminoglycoside Antibiotics in Rapidly Growing Mycobacteria

Variability in DNA Polymerase Efficiency: Effects of Random Error, DNA Extraction Method, and Isolate Type

Solution-based Circuits Enable Rapid and Multiplexed Pathogen Detection

Proximal Bacterial Lysis and Detection in Nanoliter Wells Using Electrochemistry

Optimized Templates for Bottom-Up Growth of High-Performance Integrated Biomolecular Detectors

Ipso-Hydroxylation and Subsequent Fragmentation: a Novel Microbial Strategy To Eliminate Sulfonamide Antibiotics

Tuning the Bacterial Detection Sensitivity of Nanostructured Microelectrodes

Point of Care Diagnostics: Status and Future

Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device Point-of-Care Nucleic Acid Testing For Infectious Diseases

Point-of-Care Nucleic Acid Testing For Infectious Diseases

Continuous-Flow, Rapid Lysis Devices for Biodefense Nucleic Acid Diagnostic Systems

WetLab-2: Quantitative PCR Tools for Spaceflight Studies of Gene Expression aboard the International Space Station

Ch 8: Microfluidic Diagnostics for Low-resource Settings: Improving Global Health without a Power Cord

Development Status of the WetLab-2 Project: New Tools for On-orbit Real-time Quantitative Gene Expression


Low-Cost Microdevices for Point-of-Care Testing


Refining Sample Prep for Molecular DX


Use of a Disposable Lysis Device and Capture of Unamplified mycobacterial rRNA on A Liquid Bead Array for Species Determination from Liquid Cultures


Real-Time Sample Prep on the Horizon


Novel Applications for Sample Preparation: Tool Providers Launch Innovative Products to Meet Researchers' Protein-Related Demands
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